In view of high raw material prices, and looking for cost-effective solutions, the aim of this trial was to determine if Aqualyso® STD can successfully replace dietary oils (palm oil and fish oil) in Pangasius feeds.
The trial was performed in IPB (Bogor University), Indonesia (2021).
Six isoproteic (32 % protein) feeds were prepared to gradually decrease fat content by reducing the inclusion of palm oil and fish oil (Table 1). Aqualyso® STD at 0.1% or 0.15% was added to these feeds as per Table 1. The implications on cost reduction are also presented in Table 1.
| 32%CP 7% FAT | 32%CP 6,5% FAT 0,1% AQL STD | 32%CP 6% FAT 0,1% AQL STD | 32%CP 5,5% FAT 0,1% AQL STD | 32%CP 5% FAT 0,1% AQL STD | 32%CP 5% FAT 0,15% AQL STD | |
|---|---|---|---|---|---|---|
| Soybean meal, Brazil 48% | 30,6 | 30,6 | 30,6 | 30,6 | 30,6 | 30,6 |
| Cassava chips | 15 | 15 | 15 | 15 | 15 | 15 |
| Wheat industry | 13 | 13 | 13 | 13 | 13 | 13 |
| Wheat pollard Indo | 10,33 | 10,33 | 10,33 | 10,33 | 10,33 | 10,33 |
| Meat and bone meal, 53% CP | 10 | 10 | 10 | 10 | 10 | 10 |
| Poultry by product meal, feed grade | 8 | 8 | 8 | 8 | 8 | 8 |
| Blood cell meal, ring dried | 4 | 4 | 4 | 4 | 4 | 4 |
| DDGS Indo | 3,5 | 3,5 | 3,5 | 3,5 | 3,5 | 3,5 |
| Palm oil | 2,5 | 2,25 | 2 | 1,75 | 1,5 | 1,5 |
| Fish oil | 1,2 | 0,95 | 0,7 | 0,45 | 0,2 | 0,2 |
| Premix Indo | 1 | 1 | 1 | 1 | 1 | 1 |
| Salt, NaCl | 0,5 | 0,5 | 0,5 | 0,5 | 0,5 | 0,5 |
| Aqualyso® STD | 0 | 0,1 | 0,1 | 0,1 | 0,1 | 0,15 |
| Limestone | 0 | 0,4 | 0,9 | 1,4 | 1,9 | 1,85 |
| DL_Met | 0,1 | 0,1 | 0,1 | 0,1 | 0,1 | 0,1 |
| Choline choride, 60% choline | 0,1 | 0,1 | 0,1 | 0,1 | 0,1 | 0,1 |
| Toxin binder | 0,1 | 0,1 | 0,1 | 0,1 | 0,1 | 0,1 |
| Aquavit stable C | 0,05 | 0,05 | 0,05 | 0,05 | 0,05 | 0,05 |
| Antioxidant | 0,01 | 0,01 | 0,01 | 0,01 | 0,01 | 0,01 |
| Antimold | 0,01 | 0,01 | 0,01 | 0,01 | 0,01 | 0,01 |
| 100 | 100 | 100 | 100 | 100 | 100 | |
| 2022: FO: USD 2090/ PO:USD 1670 | – USD 5 | – USD 14 | – USD 20 | – USD | – USD 31 |
Table 1: Feed formulation of the experimental diets (%)
The experimental diets were randomly assigned to 30 cages (2x1x1,5m), 5 replicates per treatment and Pangasius fish with an average size of 12 grams were stocked at 2B fish per cage. The fish were fed the experimental diets for 62 days.
Data were analyzed by a one-way analysis of variance (ANOVA). When the ANOVA identified differences among the groups, multiple comparisons were made among the means using the Tukey HSD test. Statistical significance was determined by setting the aggregate type I error to p<0.05.
1 kg of Aqualyso® STD can maintain the average daily gain and the FCR of feeds formulated down to 5% in relation to a feed formulated at 7% fat. No significant differences were found between this 7% feed and 5% feed (Fig. 1 & 2).
Based on these results, the 5.5% fat (1,75% palm oil and 0,45% fish oil) with Aqualyso® STD was the most optimal feed and was even significantly better than 7% fat. Below 5%, differences were not significant; results show a negative trend in daily gain and FCR.
Fig. 1: Average daily weight gain (g) for the different treatments after 62 days feeding the experimental diets
Fig. 2: FCR for the different treatments after 62 days feeding the experimental diets
Using a 7% fat (32% CP) as a control feed, Aqualyso® STD (1 kg/MT) can replace 0.5-2% oil inclusion (palm and fish oils) in Pangasius feeds without compromising growth and FCR.
Safe and cost-efficient replacement strategies with Aqualyso® STD are recommended at 0.5-1.5% fat replacement level.
The optimal fat replacement level is dependent on the initial/reference fat content of the feed.
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