Article published in Microbiology Spectrum – 17 September 2024
Authors:

Nuria Vieco-Saiz (Adisseo France), Damien P. Prévéraud (Adisseo France), Eric Pinloche (Adisseo France), Aurélien Morat (INRAE, Université Paris-Saclay), Pauline Govindin (INRAE, Université Paris-Saclay), Hervé M. Blottière (INRAE, Université Paris-Saclay), Elliot Matthieu (INRAE, Université Paris-Saclay), Estelle Devillard (Adisseo France), Jessika Consuegra(Adisseo France)

Unraveling the Benefits of Bacillus subtilis DSM 29784 Poultry Probiotic through Its Secreted Metabolites: An In Vitro Approach

The study investigates the probiotic Bacillus subtilis 29784 (Bs29784) and its impact on chicken intestinal health via its secreted metabolites: hypoxanthine (HPX), niacin (NIA), and pantothenate (PTH). Using in vitro models, the research reveals how these metabolites influence three key aspects of intestinal resilience: immune response, intestinal barrier, and microbiota. (See below the figure and watch the video to know more about the study)

Key findings include:

Vegetative Cells vs. Spores : Bs29784 vegetative cells are more effective in reducing inflammation compared to spores, suggesting the benefits are linked to metabolic activity.

Metabolite Effects:

PTH and NIA: Reduce pro-inflammatory pathway activation (AP-1 and NF-κB).
HPX enhances mucin production by upregulating MUC2 expression.
HPX, NIA, and PTH: Increase cell proliferation.
PTH and HPX: Improve epithelial resilience by limiting increased permeability during inflammation.

Fermentation Profiles:

In cecal fermentations, NIA increases acetate, HPX increases butyrate, and PTH increases acetate, butyrate, and propionate.
In ileal fermentations, PTH increases butyrate.
All metabolites modulate microbiota, resulting in varied fermentation patterns.

Overall, Bs29784 positively influences intestinal health by acting on the three lines of resilience: immune response, intestinal barrier integrity, and microbiota composition through its secreted metabolites.